Molecular Biology Lab Services

The Molecular Genetics Laboratory is housed in room T658. The existing facilities include: An ABI 7500 RT-PCR detector (Perkin-Elmer), PCR thermal cyclers, Nanodrop DNA analyzer, refrigerated Jouan centrifuge with rotors for Eppendorf and larger tubes, Kodak gel imager for CCD documentation, hybridization incubators for Northern and Southern analysis (Robins Scientific), horizontal and vertical gel electrophoresis units (BioRad), power supplies, and computers for data analysis. Freezers, refrigerators, an autoclave and deionized water are available in the adjoining laboratories. Additionally, the laboratory has a BSL2-level tissue culture hood and long-term sample storage at –70 degrees.

• Laboratory Equipment

Assays

• Preparing and Running Standard Agarose DNA Gels
• Electrophoresis Protocols
• RT-PCR (adapted from Promega Corp)
• SDS POLYACRYLAMIDE GEL
• 2-Dimensional IEF Gel Protocols
• Basic PCR Protocol (adapted from Promega Corp)
• Basic RT-PCR Protocol (adapted from Promega Corp)
• PCR Optimization (adapted from Promega Corp)
• RT-PCR Optimization (adapted from Promega Corp)
• Applied Biosystems Real-Time RT-PCR